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021-51875103产品中心您当前的位置：首页 > 产品中心 > 进口试剂 > 进口品牌 > Roche 11745832910DIG High Prime DNA Labeling and Detection Starter Kit I
基础信息Product information
产品名称：DIG High Prime DNA Labeling and Detection Starter Kit I
产品型号：Roche 11745832910
更新时间：2016-12-27
产品简介：
DIG High Prime DNA Labeling and Detection Starter Kit II|DNA高效地高辛标记及检测试剂盒II
产品特性Product characteristics
DIG High Prime DNA Labeling and Detection Starter Kit I
DIG High Prime DNA Labeling and Detection Starter Kit I|DNA高效地高辛标记及检测试剂盒II|地高辛标记试剂盒|高效地高辛标记试剂盒|罗氏地高辛标记试剂盒
DNA高效地高辛标记检测试剂盒I（罗氏Roche 货号：11745832910）
DIG-High Prime DNA Labeling and Detection Starter Kit I | |
Kit for color detection with NBT/BCIP. | |
1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 µg DNA per assay, blots of 100 cm2
Application
DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.
Product Description
Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
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