ELISA AP Substrate
Lumigen APS-5 is a proprietary acridan based chemiluminescent substrate for ELISA detection of alkaline phosphatase (AP) conjugated molecules. The unique acridan chemistry of provides temperature insensitive light output and more rapid peak intensity as compared to the dioxetane based AP substrates, Lumi-Phos 530 and Lumi-Phos Plus.
- Excellent sensitivity - low picogram to femtogram
- Rapid peak intensity - reduced assay time and increased throughput
- Temperature insensitive - analytical results are insensitive to temperatures from 22° C - 35° C reducing the need for precise temperature control
- Sustained luminescence - timing is less critical with light intensities able to be read at any time to produce linear calibration curves
Rapid Peak Intensity
The light-output peak intensity of reaches sustained maximum within seconds of substrate addition. Light emission is maximal at 450 nm.
